You decide to use different bacterial strains (each having one protein of the re

Question

You decide to use different bacterial strains (each having one protein of the replication machinery mutated) in order to examine the role of individual proteins in the normal process of DNA replication.

What part of the DNA replication process would be most directly affected if a strain of bacteria lacking helicase were used to make the cell extracts?

a. initiation

b. Okazaki fragment synthesis

c. leading - strand elongation

d. lagging - strang completion

What part of the DNA replication process would be most directly affected if a strain of bacteria lacking single - strand binding protein were used to make the cell extracts?

a. initiation of DNA synthesis

b. Okazaki fragment synthesis

c. leading - strand elongation

d. lagging - strand completion

What part of the DNA replication process would be most directly affected if a strain of bacteria lacking DNA ligase were used to make the cell extracts?

a. initiation of DNA synthesis

b. Okazaki fragment synthesis

c. leading - strang elongation

d. lagging - strand completion

Explanation / Answer

1)

DNA primase is required for initiation of replication process hence lacking DNA primase enzyme affects initiation of DNA replication. Option a is correct.

2)

After the primase is bound to complentary sequence, the single strand binding proteins (SSB proteins) are associated with primase complex to start the replication process. If SSB proteins are lacking, then strand elongation process is affected. Option c is correct.

3)

DNA ligase is the enzyme that joins the DNa fragments (okazaki fragments of lagging strand) to complete the strand. If DNA ligase is lacking, then lagging strand completion is affected. Hence, option d is correct.

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