You find a transcription activator called Msn2 in yeast. You do a deletion analy

Question

You find a transcription activator called Msn2 in yeast. You do a deletion analysis to dissect out the function of each part of the protein. When you delete the N-terminus you get no DNA binding or transcription of a reporter for Msn2 activity. When you delete the C-terminus, you get DNA binding but no transcription. You get lots of reporter activity with the wild type protein with no deletion. Which of the following statements is true?

The reporter you are using isn't activated by Msn2.

You only need the the activation domain for this transcriptional activator to work.

The N-terminus contains the activation domain of the transcriptional activator

The N-terminus contains the DNA binding domain of the transcriptional activator

The reporter you are using isn't activated by Msn2.

You only need the the activation domain for this transcriptional activator to work.

The N-terminus contains the activation domain of the transcriptional activator

The N-terminus contains the DNA binding domain of the transcriptional activator

Explanation / Answer

Based on the given data, the correct option is (D) the N-terminus contains the DNA binding domain of the transcriptional activator. Because the deletion of N-terminal results the loss of DNA binding or transcription of a reporter, but the C-terminal deletion made the transcription activator Msn2 to bind on the DNA.

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