Questions 8-10. To the right is data from an experiment in which three different
ID: 37552 • Letter: Q
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Questions 8-10. To the right is data from an experiment in which three different yeast strains have been labeled with 3H thymidine. Samples from each of those cultures have been isolated at different time points, captured on a filter and counted so that the total amount of radioactivity in each sample can be measured. 8. The yeast strain labeled with the X's is: a) a yeast strain with a non functional primase b) a yeast strain with a non functional topoisomerase c) a yeast strain in which the 5' to 3' editing function of DNA polymerase is nonfunctional 9. The yeast strain labeled with the closed circles is: a) a yeast strain with a non functional primase b) a yeast strain with a non functional topoisomerase c) a yeast strain in which the 5' to 3' editing function of DNA polymerase is nonfunctionalExplanation / Answer
8. b. topoisomerase is non functional - Topoisomerases are enzymes that regulate the overwinding or underwinding of DNA. The winding problem of DNA arises due to the intertwined nature of its double-helical structure. During DNA replication and transcription, DNA becomes overwound ahead of a replication fork. If left unabated, this tension would eventually stop the ability of the enzymes involved in these processes to continue strand extension down the DNA strand.
9. c. a yeaststraiin in which the 5' to 3' editing function of DNA polymerase is nonfunctional -DNA polymerase I 5' to 3' exonuclease activity and also has 3' to 5' and, which is used in editing and proofreading DNA for errors.The 5' to 3' activity can remove mononucleotides or up to 10 nucleotides at once but, The 3' to 5' only remove one mononucleotide at once. as this is non functional errors are overlooked and replication halts at one point
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