Problem 1 (15 points) You are given the following DNA sequence, which is believe

Question

Problem 1 (15 points)

You are given the following DNA sequence, which is believed to contain a small protein-coding gene.

GGAGGCGTAA AATGCGTACT GGTAATGCAA ACTAATGG

If this sequence is fully transcribed (used as a coding strand), what is the corresponding mRNA sequence?

Which region of the mRNA do you think can be translated into a protein (hint: Can you identify the start codon and stop codon from the mRNA sequence?)

What is the protein sequence encoded by the gene?

If the reverse-complementary strand of the DNA sequence is also tran-

scribed, what will be the mRNA sequence?

Do you think this reverse-complementary strand can encode a protein by itself ?

Explanation / Answer

The corresponding mRNA is : GGAGGCGUAA AAUGCGUACU GGUAAUGCAA ACUAAUGG . It is a copy of given DNA sequence, with T’s substituted by U’s. AUG CGU ACU GGU AAU GCA AAC UAA Met - Arg - Thr - Gly - Asn - Ala - Asn 5’-CCATTAGTTT GCATTACCAG TACGCATTTT ACGCCTCC-3’ [Reverse the strand after writing down the complementary base] It cannot encode a protein as the reverse complementary strand has no start and stop codons..

Related Questions

Navigate

• Browse (All)
• Browse P
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.