Reade o C view.php?id 1551587 -University of Cincinnati CHEM 3040 Spring 15 MERI

Question

Reade o C view.php?id 1551587 -University of Cincinnati CHEM 3040 Spring 15 MERINO: Chp 5 Hw mod biscm sapling learning order the steps of the Southern blot procedure as they would be applied to DNA fingerprinting analysis. First step Last step Incubate membrane with radioactively labeled ssDNA probe. Cleave DNA using restriction endonucleases. Transfer ssDNA to a nylon or nitrocellulose membrane. Denature DNA by soaking gel in NaOH. Separate DNA fragments by gel electrophoresis. Place film onto membrane to produce autoradiogram

Explanation / Answer

1. Cleave DNA using restriction endonucleases.

2. Separate DNA fragments by gel electrophoresis.

3. Denature DNA by soaking gel in NaOH.

4. Transfer ssDNA to a nylon or nitrocellulose membrane.

5. Incubate membrane with radioactively labeled ssDNA probe.

6. Place film onto membrane to produce autoradiogram.

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