Assume you need to insert a human gene into a bacteria. You need the protein fro

Question

Assume you need to insert a human gene into a bacteria. You need the protein from the human gene to be expressed in the bacteria. There are six potential gene constructs you could use to try to insert the human gene into the bacteria, they are labeled A) ? F). In this figure, G-PPP- is a 5?-cap, and ?- AAAAA? is a poly-A tail. exon intron exon intron exon 1. Which of these six gene images is the correct representation of the gene that should be put into the bacteria to produce the same protein as in a human. 2. Explain your answer to question 1. 3. Once inserted, do you expect that the nucleotide sequence that codes for the human protein will produce a protein with the same amino acid sequence in the bacteria? 4. Explain your answer to question 3.

Explanation / Answer

1) Image D is appropriate. It represents cDNA, it seems

2) Prokaryotes do not process introns Hence intron free cDNA can be used for heterologous expression.

3) it will produce human protein, but there will be some dicrepancies.

4)Transcription and translation rate will be less efficient due to the presence of human gene in bacteria. But codon optimization stategy can be used for better heterologous expression.

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