Assume you need to insert a human gene into a bacteria. You need the protein fro

Question

Assume you need to insert a human gene into a bacteria. You need the protein from the human gene to be expressed in the bacteria. There are six potential gene constructs you could use to try to insert the human gene into the bacteria, they are labeled A) - F). In this figure, G-PPP- is a 5?-cap, and -AAAAA is a poly-A tail. 1. Which of these six gene images is the correct representation of the gene that should be put into the bacteria to produce the same protein as in a human. 2. Explain your answer to question 1. 3. Once inserted, do you expect that the nucleotide sequence that codes for the human protein will produce a protein with the same amino acid sequence ?n the bacteria? 4. Explain your answer to question 3.

Explanation / Answer

1. E

2. The sequence has exons along with a cap and AAAAA tail. In bacteria, post transcripptional changes wont occur. So, capping and poly AAAAA tail wont be added naturally. That is why we need to put this in our construct. Splicing also won't happen in bacteria, so we choose already spliced construct.

3. Yes

4. genetic code is same in humans and bacteria. So, the insert will produce the same amino acid sequence as of human. But, there won't be post translational modifications. If the secreted protein will be active or not we are not sure and acquiring right conformation also depends on post tranlational modifications whihc won't take place in bacteria.

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