A. In vitro transcription reactions were performed using a DNA template containi

Question

A. In vitro transcription reactions were performed using a DNA template containing a Rho independent

terminator and wild type (RpoB+) or rpoB8 mutant RNA pol. The length of the RNA transcript will be

approximately 6100 nt when RNA polymerase encounters the terminator.

- How long does it take until the wild type and rpoB8 polymerases to reach the terminator?

- What is the elongation rate of the wild type and rpboB8 polymerases? Show your calculation in nt/sec.

- Explain which polymerase terminates more efficiently.

Explanation / Answer

it takes 35 mins for the wild type to reach the terminator and 40 mins for mutant to reach terminator.

-

the wild type polymerase terminates more efficiently as compared to the mutant, because the exit channel of the mRNA from the RNA polymerase is blocked by the rpoB8 mutation.

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