A student wishes to target hexokinase, a cytosolic enzyme of glycolysis, to the

Question

A student wishes to target hexokinase, a cytosolic enzyme of glycolysis, to the mitochondria of cells in culture as a project for a senior honor's thesis in her college. What experiment would be necessary to accomplish this task?

A) The student would need to clone the codons for a stop-transfer sequence into the gene for hexokinase in these cells.

B) The student would need to mutate and cause a defect in the gene for the signal recognition particle in these cells.

C)The student would need to clone a transit sequence into the gene for hexokinase into these cells.

D)The student would need to clone the codons for a start-transfer sequence into the gene for hexokinase in these cells.

Explanation / Answer

Clone is a genetically identical copy of an organism. It may be naturally occurring or created in the lab. There are different types of cloning mechanisms; most commonly used method is rDNA technology. During the process of rDNA technology foreign DNA molecule introduced into target cells through the vector ( i.e. Plasmid , viral or gene gun ) . This procedure is most commonly used in gene transfer strategies and gene expressions studies.

So that, we need to clone a transit sequence into the gene for hexokinase into these cells. That is a correct way accomplish this task

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