Assume that the hypothetical compound below can be metabolized to yield 1 succin

Question

Assume that the hypothetical compound below can be metabolized to yield 1 succinyl CoA and 1 acetoacetate. Pridoxal-phosphate is involved, but an aminotransferase reaction does not occur. Use structural formulas and give the mechanism(s) of the portion(s) of the reaction sequence in which pyridoxal 3. phosphate is involved. Indicate which, if any, coenzymes are needed in the other reactions of the sequence. (HINTS: Start with 2 PLP-catalyzed reactions. Some nonenzymatic steps will be required. After these steps, what amino acid does the carbon skeleton resemble?) OS H CH, COO CH, OH NH

Explanation / Answer

PLP enzymes catalyze a big number of biochemical reactions. scope of PLP-catalyzed reactions seems to be greatly vast .
There is a simple uniform principle :
in a resting state PLP is covalently bonded to the amino group of an active site lysine , which forms an internal aldimine. as the amino substrate interacts with the active site, production of new Schiff base takes place , commonly mention as the external aldimine . for each PLP-catalyzed reaction mechanistic pathway diverges.To investigate the transimination reaction Density functional methods have been applied after this stage, and the results have shown that the reaction involves 3 sequential steps-:

(i) tetrahedral intermediate with the the amino substrate and active site lysine bonded to the PLP co-factor been formed

(ii) between the amino substrate and the lysine residue non-direct proton transfer took place ; and

(iii) after the dissociation of the lysine residue , formation of the external aldimine occurs.

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