What is a good suggestion to improve the outcome of column chromatography to sep

Question

What is a good suggestion to improve the outcome of column chromatography to separate the components of spinach? -Identifier" list of the colored fractions collected (i.e. test tubes containing colored eluent). you were can be A, B, C, etc. color" is the color of the fraction, "eluent"is the solvent the using to elute that colored fraction, and "predicted identi is what you expect pigment(s) in that fraction to based on the laboratory manual procedure. For example, based on the lab manual you expect your first colored fraction A elute with hexanes/ethyl acetate, and to be B-carotene. However, record your actual data here. Eluent Predicted Identity 90:lo hexanes; einrl B-carotene. yellow acetate. Teen 70:30 kerares ety acerele Chlorophyll fure ethyl aut tate Xanthophy as yellow 2. sketch the TLC for your column's colored fractions. Plates Two templates are given below, just in case one TLC plate was not enough. Label the origins using the identifiers in your above list; the original extract can be labeled "X". Also show the solvent front at the top of the plate. a Control t S. can S. am A 1.6 Ne breen 2,7 en T B 2.5 cm 2.2 em yellow 2.1 cm x 1.0 en

Explanation / Answer

(i) Hexane is a non-polar solvent and ethyl acetate is polar solvent. 90:10 hexane:ethyl acetate means the solvent is more non-polar. When this solvent is used, it eluted beta-carotene. So, beta carotene would be also non-polar. If we use only hexane as solvent, we will get pure beta-carotene. Spots of other compounds in the sample will not be obtained.

(ii) After we elute beta-carotene completely, we will add mixture of hexane: ethyl acetate in ratio of 80:20. More of hexane will be used, because ethyl acetate will start eluting xanthophyll also. To get pure chlorophyll, 80:20 mixture of solvent will be used.

(iii) To elute xanthphyll, pure ethyl acetate will be used.

Related Questions

Navigate

• Browse (All)
• Browse W
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.