1. Micropipetting, Dilutions, and Significant Figures. Seven hundred fifty micro

Question

1. Micropipetting, Dilutions, and Significant Figures. Seven hundred fifty microliters of 200.0 µM NAD+ is added with an Eppendorf Research Plus P1000 to a microcentrifuge tube, followed by two hundred microliters of water added with a Eppendorf Research Plus P200. What is the concentration of the resulting solution using the correct number of significant figures? Show your work.

2. Micropipetting, Dilutions, and Significant Figures. One hundred forty-seven microliters of water is added with an Eppendorf Research Plus P200 to a microcentrifuge tube, followed by two microliters of 500.0 µM NAD+ added with a Eppendorf Research Plus P20. What is the concentration of the resulting solution using the correct number of significant figures? Show your work.

Explanation / Answer

1. Concentration = moles/ volume

So, given moles = molarity x volume = 200.0microM x 750microliters= 150000moles = 0.15micromoles

So, volume diluted = 200microL

Total volume = 750+200 = 950microL

So, concentration = moles/molarity = 0.15micromoles/960microL = 1.5789e-4M = 157.89microM

So, concentration of solution = 157.9microM ( 4 significant numbers, same as in 200.0microM has 4 sig. figures)

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