a) A temperate bacteriophage has the gene order x1 x2 x3 att x4 x5. What is the

Question

a) A temperate bacteriophage has the gene order x1 x2 x3 att x4 x5. What is the gene order of the prophage

b) Can the lytic P1 phage infect the lysogen produced by integration of the phage described in question a? Explain your answer. (Hint: The phage in part a is Lambda)

c)A new series of rII mutations in the virulent phage T4 has been isolated, and the results of phage recombination experiments between all twelve of the new mutations are listed below:

Draw a linear genetic map showing the location of all point mutations and the frequency of recombination between adjacent mutations. Describe the location and extent of all deletions.

Explanation / Answer

a)

Based on the given data, the gene order is as follows:

x1 x4 x2 x3x5

b)

Yes, the lytic P1 phage infects the lysogen produced by integration of the phage, because the page genome has integration sites.

c)

5, 12, 9, 10, 2, 11, 4, 7, 6, 8, 12, 3, 1

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