b) Estimate the values for these important constants: What is Vmax? I\'ve tried

Question

b) Estimate the values for these important constants:

What is Vmax? I've tried 200 and 100 and neither work. Km is right.

The Michael equation is often used to describe the kinetic characteristics of an enzyme-catalyzed reaction maxls] Where vis the velocity or rate, V s the maximum velocity, Kim is the Michaelis Menten constant, and [S] is the substrate concentration (MM/min) a) A graph of the Michaelis-Menten equation is 300 a plot of a reaction's initial velocity (Vo) at 275 different substrate concentrations (IS]) 1/2 Vmax First, move the line labeled "Vr to a position that represents the maximum velocity of the enzyme. 75 Next, move the line labeled "1/2 V Michaelis Menten Curve 150 Km to its correct position. Then, move the line labeled "Km" to its 100 correct position. 75 10 20 30 40 50 60 70 80 90 100 IS) (AM) b) Estimate the values for these important constants Number Number Km 14 UM UMI min

Explanation / Answer

The given graph predicts that reaction velocity is the function of substarte concentration

From the graph we can understand that the substrate concentration remains a constant at175mM

Since Vmax represents the maximum velocity achied by the system at maximum subtrate concentrations, alternatively we can take were the curve flattens out as the maximum concentration 175mM/min

Once this is clear we can move the 1/2 Vmax line to 175/2= 87.5 after which if we move the Km line it correctly touches 14mM which is your calculated answer

Related Questions

Navigate

• Browse (All)
• Browse B
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.