5. A. A new analytical extraction method was developed for a DNA analysis in fin

Question

5. A. A new analytical extraction method was developed for a DNA analysis in finger print samples. A finger print sample was analyzed six times using the new protocol and found to contain average value of 7580.0 g/L with a standard deviation of 10 for DNA. A spike recovery was conducted to validate the accuracy of the new method. A spike of the blood with 150.0 g/L DNA gave a concentration of 7725 g/L.

A. What is the percentage recovery of DNA spike recovery study?

B. Based on the result of percentage recovery, will you consider the method accurate for DNA extraction?

C. Assuming the “reference value” of DNA in the samples, is there any significant difference between the result of the finger print analysis and the reference values at 99.9% confident interval?

Explanation / Answer

a) % recovery =100* (7725-7580)/150 = 96.67 %

b) Yes, since the recovery % is high

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