1) Sickle cell anemia occurs in individuals homozygous for autosomal recessive a

Question

1) Sickle cell anemia occurs in individuals homozygous for autosomal recessive allele Hb^S. Analysis of Southern blots of human DNA cut with the enzyme Hpal and probed with a radioactively labeled B-globin cDNA revealed that HpaI does not cut within the B-globin gene. However, positions of HpaI sites surrounding the gene vary among individuals, producing at least 3 RFLPs in the population. The sizes of these RFLP alleles are 13, 7.6 and 7 kb. The pedigree below illustrates a family in which two individuals have sickle cell anemia (shaded in black). Also shown are the results of a Southern Blot in which DNAs from the individual in this family were cut with HpaI, run on a gel, transferred to a filter, and probed with radioactively labeled B-globin cDNA.

Which RFLP allele is associate with the Hb^S allele in individual I-2 in the pedigree?

a) 13 kb
b) 7.6 kb
c) 7 kb
d) Both the 7.6 and 7 kb allele

Which RFLP allele did individual II-3 inherit from his mother?
a) 13 kb allele
b) 7.6 kb allele
c) 7 kb allele
d) both the 7.6 and 7 kb allele

Figures for Quiz 5 Use the following description and figures for questions 1-2. Sickle cell anemia occurs in individuals for the autosomal recessive allele Hbs. Analysis of Southern blots of human D probed with a radioactively labeled -globin cDNA revealed that Hoal does not cut within the -globin gene. How positions of Hoal sites surrounding the gene vary among individuals, producing at least three RFLPs in the popul The sizes of these RFLP alleles are 13, 7.6, and 7 kb. The pedigree below ilustrates a family in which two individ have sickle-cell anemia (shaded in black). Also shown are the results of a Southern blot in which DNAs from the individuals in this family were cut with Hoal, run on a gel, transferred to a filter, and probed with radioactively label -globin cDNA. nd figures for questions 1-2. Sickle cell anemia occurs in individuals homozy NA cut with the enzyme Hoal and I-1 I-2 II-L II-2 II-3 IL-4 -C) 13 kb 2 7.6 kb | 7 kb 2 3 4

Explanation / Answer

1. RFLP allele is associate with the Hb^S allele in individual I-2 in the pedigree is a) 13 kb

2. RFLP allele individual II-3 inherited from his mother is b) 7.6 kb

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