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In the plant Arabidopsis thaliana , a mutant phenotypecalled twisted leaves may

ID: 4829 • Letter: I

Question

In the plant Arabidopsis thaliana, a mutant phenotypecalled twisted leaves may be caused by either a chloroplast gene(CT = normal; Ct = twisted)or a recessive nuclear gene (NT = normal;Nt = twisted). Chloroplasts in these plants arematernally inherited. Predict the genotypic and phenotypic ratiosin the offspring of the following crosses. Note: Normal leavesare dominant. Therefore, even one normal allele is enough to givenormal leaves.

i) CT NtNt female ´ CtNTNT male

ii) Ct NtNt female ´ CTNTNT male

iii) F1 female from cross (i) ´ F1 male from cross (ii)

iv) F1 female from cross (ii) ´ F1 male from cross (i)

Explanation / Answer

CT allele=normal;           CT CT= normal   CT Ct= normal

Ct = twisted or arecessive nuclear gene

Chloroplasts in these plants are maternallyinherited

NT allele = normal;NT NT = normal;NT Nt =normal;

Nt = twisted.recessive gene

               CT NtNtfemale x                    Ct NTNTmale

Gametes          CTNt                                             Ct NT

                           CT  Ct NT Nt      -------------   offspring  

So               CT   Ct NT Nt       ----------normal

ii)                          Ct NtNt female x                    CTNTNT male

            Gametes      CtNt                                                     CT NT

                                       CT Ct NTNt          normal

iii) F1 female from cross (i) ´ F1 male from cross (ii)

               CTNtNt female x           CT NTNT male

Gametes          CTNt                                    CT NT

                 CT Nt     CTNT         -normal

iv) F1female from cross (ii) ´ F1 male fromcross (i)

             Ct NtNtfemale x          Ct NTNT male

Gametes ---------     CtNt                                             CtNT

                      Ct Nt    CtNT -normal

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