Enzyme denaturation with temperature Calculate the value of the denaturation con
ID: 483546 • Letter: E
Question
Enzyme denaturation with temperature
Calculate the value of the denaturation constant kd for a given enzyme, using the equation provided from textbook:
kd=Ad*e^(-Ed/RT)
where Ad=3x10^47 and Ed=70 kcal/g mole (use a temperature of 41°C). Then, what is the decrease in enzyme activity due to denaturation, if the enzyme is exposed to a temperature of 41°C for a period of 5 minutes, or alternately a period of 20 minutes? Similarly, what is the decrease in enzyme activity due to denaturation, if the enzyme is exposed to a temperature of 46°C for a period of 5 minutes, or alternately a period of 20 minutes?
Explanation / Answer
(a): Given Ad = 3x1047
Ed = 70 kcal/g mol = 70 x (1000 cal / 1 kcal) x (4.184 J / 1 cal) x kcal / gmol = 2.93x105 J/gmol
Temperature, T = 41 C = 41 + 273 = 314 K
Denaturation constant, kd = Ad*e^(-Ed/RT)
=> kd = 3x1047 x e^( - 2.93x105 J.gmol-1 / 8.314 J.mol-1K-1 x 314 K)
=> kd = 0.0568 (answer)
(b):
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