Enzyme assay reaction tubes. Before proceeding to the next step of initiating th

Question

Enzyme assay reaction tubes. Before proceeding to the next step of initiating the reactions by adding the dopa, use the formula C_1 V_1 = C_1V_2 to determine what the final concentration of dopa will be in each tube and enter in the table. Also before initiating the reaction, consider the expected results for each tube. What do you expect to happen to the absorbances in the 'control' tubes (odd numbered tubes)? Which tubes do you expect to see the biggest increase in absorbance? Smallest increases?

Explanation / Answer

C1 =40mM =[dopa]

V2= Vtotal = Venzymedilution+Vbuffer+V1+Vto initiate reactionbuffer= Vfinal volume = 5mL

C1=N1/V1 = dopa moles/V1

C2=N2/V2 = dopa moles/V2

N1 = C1V1, N2=C2V2

N1=N2 Then C1V1=C2V2

C2=C1V1/V2

For tube 1:

C2=40mM *0mL /5mL = 0

For tube 2:

C2=40mM *0.5mL /5mL = 4mM

V1 mL V2 mL C2 mM 1 0 5.0 0 2 0.5 5.0 4 3 0 5.0 0 4 0.5 5.0 4 5 0 5.0 0 6 0.5 5.0 4 7 0 5.0 0 8 0.5 5.0 4

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