compare atomic spectroscopy and atomic emission need help with these two 6. hy i
ID: 484356 • Letter: C
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compare atomic spectroscopy and atomic emission need help with these two 6. hy is atomic emission more sensitive to flame instability than atomic absorption? 7.A 5.00-ml sample of blood was treated with trichloroacetic acid to precipitate the proteins. After centrifugation, the resulting solution was brought to a pHof 3and was extracted with two 5.0 ml portions of methyl isobutyl ketone containing the organic lead complexing agent APcD. e extract was aspirated directly into an air-acetylene flame yielding absorbance of 0.44 at 3 nm. Five milliliter aliquots of an standard solutions containing 0.35 and 0.55 ppm Pb were treated in the same way and yielded absorbances of 0.396 and 0.599. Calculate the concentration of Pb (ppm) in the sample assuming that Beer's law is observedExplanation / Answer
6) Atomic emission is more sensitive to flame instability because optimum excitation conditions vary from element to element. Some elements needed high temperatures for excitation and others needed low temperatures. Atomic emission is the technique that will be severely affected by fluctuations in temperature, because signal is dependent on the number of atoms in the excited state. This number is significantly affected by fluctuations in temperature as derived from the Boltzmann equation. In atomic absorption, the signal depends on the number of atoms in ground state that will absorb energy. The number of atoms in ground state is always very high as related to the number of excited atoms. There are a very high number of atoms in the ground state even at high temperatures. So, atomic absorption will not be affected to any significant extent by fluctuations in temperature, if compared to atomic emission spectroscopy.
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