For Microbiology, I am doing a lab and am stuck on two of the questions. It is 1

Question

For Microbiology, I am doing a lab and am stuck on two of the questions. It is 1-4 Common Aseptic Transfers and Inoculation Methods.

1) Which medium was most difficult for you to transfer from? Which medium was most difficult for you to inoculate? Explain your difficulties. (Note: there are no current answers to these questions, but based on your evaluation of personal experiences)

2) Did you get growth on/in the sterile NB and NA slant tubes you practiced with? If so, where did you see it and what might have been its source(s)?

Explanation / Answer

1) The solid slant medium was most difficult to transfer from and inoculate because it required quick transfer and inoculation and the colonies were to be picked with utmost care so that the surface of the growth medium did not break.

2) The source of contamination could be improper sterilization of the medium or environmental contamination during inoculation.

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