Chg DNA Technologies 2127/201706:00 PM al100 e Print calculator Periodic Table 1

Question

Chg DNA Technologies 2127/201706:00 PM al100 e Print calculator Periodic Table 1 of 18 A DNA sample and a plasmid with the ampR and lacz (for the enzyme genes are treat with EcoRI with goal making recombinant DNA molecules, the lacz gene is region so that it is disrupted DNA inserts. Put the remaining steps for this process in the correct order Not all statements will be used. Note: If you answer any part of this question a single red X wiWappear indicating that one or more of the phrases are placed incorrectly. DNA is treated with restriction enzyme. Blue colonies that survive the culture medium contain the recombinant plasmid DNA ligase sample and the plasmid. AI the fragments have sticky ends 5-AATT 3 cells containing the recombinant plasmid ampicilin and X-gal (agalactose derivative) white colonies that survive on culture medium are as having the recombinant plasmid Colonies containing the recombinant A Previous Give up view solution Check Answer Next aExt-L terms of use 2011-2017 sapling earm

Explanation / Answer

Step 1) DNA is treated with restriction enzyme.

Step 2)-All the fragments have identical sticky ends: 5'-GGCC-3'.

Step 3)-DNA ligase joins the sticky ends of the DNA sample and the plasmid.

Step 4) Cells containing the recombinant plasmid are grown in a medium with ampicillin and x-gal (a galactose derivative).

Step 5) White colonies that survive on the culture medium contain the recombinant plasmid.

Step 6) Colonies containing the recombinant plasmid can be removed from the culture medium and grown.

Related Questions

Navigate

• Browse (All)
• Browse C
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.