Calculate the Rf values for compounds X, Y, and Z on the following TLC plates. W

Question

Calculate the Rf values for compounds X, Y, and Z on the following TLC plates. Which development solvent do you think gave the best results, briefly explain? 50% Ethyl Acetate/50% Hexane 10% Ethyl Acetate/90% Hexane Hexane A mixture of X, Y, and Z from the previous problem was to be separated using column chromatography. The TLC results are a good predictor of how the column will work. If the mixture is eluted with 10% ethyl acetate in hexane, which compound would >e expected to come off the column first, which one will be last?

Explanation / Answer

Rf value for X/Y/Z= Distance travelled by X/Y/Z from base line/ Distance travelled by solvent from base line

Rf value is always less than oneor equal to one. Here you have not supplied distance travelled.

So just add your observation to above equation and solve it.

The solvent system B is best because it is giving maximum separation between x,

y and z

X has highest Rf value so it will be eluted first.

Elution order will be X>Y>Z

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