The absorption spectra for chlorophyll a and chlorophyll b are plotted below on

Question

The absorption spectra for chlorophyll a and chlorophyll b are plotted below on the same axis. If these were a mixed sample, how could you determine which is which? If these were mixed how might you only get chlorophyll a and prove that you were correct in only having chlorophyll a?

Questions to answer: The absorption spectra for chlorophyll a and chlorophyll b are plotted below on th these were a mixed sample, how could you determine which is which? If these w might you only get chlorophyll a AND prove that you were correct in only having Chlorophyll a Cruorophyli b Wavelength inm) Yellow Orange Absorption spectra 2. Calculate the extinction coefficient (g) of the standards. The extinction coefficient (g) in the Beer-Lambert equation is a measur mpound in solution light at a nartirulnr

Explanation / Answer

UV-Vis spectra of chlorophyll

Chlorophyll b absorbs at shorter wavelength as compared to chlorophyll a. Thus among the two peaks, the one appearing before is for chlorophyll b round 645 nm and the one after is chlorophyll a around 660 nm.

To confirm this, one may add a standard sample of chlorophyll a or chlorophyll b, one at a time and see the increase in intensity of the peak with each addition. This will proove which peak corresponds to which chlorophyll.

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