20. Place the following steps in cloning DNA in the correct order 1 - use vector
ID: 50237 • Letter: 2
Question
20. Place the following steps in cloning DNA in the correct order
1 - use vector to deliver new rDNA to bacterial or other cells
2 - use restriction enzymes to cut a plasmid and add the desired gene
3 - isolate and cut out a desired gene using restriction enzymes
4 - use DNA ligase to seal the new gene
5 - allow bacterial cells to replicate and produce desired product
A) 1, 2, 3, 4, 5
B) 5, 3, 2, 4, 1
C) 2, 5, 3, 1, 4
D) 3, 2, 4, 1, 5
A) 1, 2, 3, 4, 5
B) 5, 3, 2, 4, 1
C) 2, 5, 3, 1, 4
D) 3, 2, 4, 1, 5
Explanation / Answer
D) 3, 2, 4, 1, 5
The first step in cloning involves using restriction enzyme so that desired gene is cut and isolated for cloning. After that restriction enzyme is used to cut plasmid and insert the gene of interest in it. The insert is then sealed using DNA ligase to give rDNA. This rDNA is then delivered to bacterial cells with the help of vector. Once the rDNA is delivered bacterial cells replicate and give product as desired.
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