In take up order from the environment? Improve them selves, var, o c. does y the

Question

In take up order from the environment? Improve them selves, var, o c. does y the transformation solution contains calcium chloride? t nna eso the Dembrane. acess ca bl Dilution problems (10 points) a. (YOU MUST How ALL LATIONS FOR FULL CREDIT) KCI You have 2 M KCI stock solution, you want to make 100ml 200mM (by adding distilled water). Calculate the amount of is required to make the desired solution? How much water will you add to make the required solution? (4 points) 2 x 20 and go mi of water b. If you want to make a buffer containing 200mM KCI and 20% methanol in a final volume of 200ml. The stock solutions are 2M KCI and 100% methanol. How much of each ingredient you have to take from the stock solution? Also calculate the distilled water you need. (6 points) .2 x loo 20 TROO I- 2 M 200? ICO. & O m.

Explanation / Answer

a)

Apply Dilution law:

M1*V1 = M2*V2

stock solution is 1, desired solution is 2

so

2M*V1 = (200 mM)(100 mL)

change mM to millimol

2M*V1 = (200*10^-3 M)(100 mL)

solve for V1

V1 =  (200*10^-3 M)(100 mL) / 2

V1 = 10 mL required from stock solution

b)

buffer for:

200 mM KCl

20% methanol

Final V = 200 mL

so...

for a final V = 200 mL

20% must be methanol so

20/100*200 = 40 mL of methanol required

for KCl mix--

V = 200 mL

so

M1*V1 = M2*V2

V1 = M2/M1*V2 = (200*10^-3)/(2)(200) = 20 mL

so

Vtotal =20 mL of KCl + 40 mL of methanol + 140 mL of water

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