Select the representative data for the oil that you used from the table above. I

Question

Select the representative data for the oil that you used from the table above. If your oil is not listed, use the representative data for olive oil. The sample data was obtained by placing 5 drops of oil into a 25 mL volumetric flask and diluting to the mark. Assume that the mass of oil placed in the flask was 0.2185 g (approximated by assuming that 5 drops is 0.25 mL and using the density of methyl oleate). From the peak area for inethyl oleate (don't forget to first convert to 10^6 to match the calibration curve!), you can use the equation of the line to solve for the concentration of the injected sample. Use the volume of the sample to convert the concentration to mols, and then grams of methyl oleate.

Explanation / Answer

Fo the given olive oil injection

Area = 209.978362 x 10^6

Feed this in place of y in the equation for the graph

y = 16.263x + 1.909

209.978362 = 16.263x + 1.909

x = 12.794

with R^2 = 0.992

The concentration becomes = 12.794 x 0.992

                                             = 12.692 umol/ml

concentration of methyl oleate in moles = 12.692 x 10^-6 mol x 25/0.25

                                                               = 0.00127 mol

concentration of methyl oleate in grams = 0.00127 mol x 296.5 g/mol

                                                                = 0.376 g

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