The following questions apply to separation of a solution containing pentanol (F
ID: 511016 • Letter: T
Question
The following questions apply to separation of a solution containing pentanol (FM 88.15) and 2, 3-dimethy1-2-butanol (FM 102.17). Pentanol is the internal standard. Separation of a standard solution containing 229 mg of pentanol and 235 mg of 2, 3-dimethyl-2-butanol in 10.0 mL of solution led to a pentanol:2, 3-dimetnyl-2-butanol relative peak area ratio of 0.930:1.00. Calculate the response factor, F, for 2, 3-dimethyl-2-butanol. Number _______ Calculate the areas for pentanol and 2, 3-dimethyl-2-butanol gas chromatogram peaks in an unknown. For pentanol, the peak height was 75.0 mm and the width at half-height was 4.4 mm. For 2, 3-dimethyl-2-butanol, the peak height was 97.2 mm and the width at half-height was 2.7 mm. Assume each peak to be a Gaussian. Pentanol: Number _______ mm^2 2, 3-Dimethyl-2-butanol: Number _______ mm^2Explanation / Answer
(a) Response factor F
F = (area/concentration)2,3-dimethyl-2-butanol/(area/concentration)pentanol
= (1/235)/(0.930/229)
= 1.05
(b) Area for pentanol = 1/2(75 x 4.4) = 165 mm^2
area for 2,3-dimethyl-2-butanol = 1/2(97.2 x 2.7) = 131.22 mm^2
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