1. List six factors that will influence your decison in recommeding methods of e

Question

1. List six factors that will influence your decison in recommeding methods of extraction of fermentation products.
2. Lis four methods used for separation of solids. Is separation of solids and from liquid always necessary before extraction of fermentation products?
3. List four methods of mechanical disruption of cells to harvest intracellular products.
4. List three non-mechanical methods of cell disruption and give specific examples.
5. Give example of chemical agents that can be employed to extract fermentation products.
6. List four methods of chromatography for purification of fermentation products.
7. What methods would you recommend for final processing of fermentation products before packging.

Explanation / Answer

Based on the given data,

1)

The factors that will influence on in recommending methods of extraction of fermentation products are:

Chemical nature of product (acid or base)

Solubility of product

Reactivity of product with other reagents

Sedimentation coefficient of product
Nature of solvent, which is used for product extraction

pH, temp, etc

  2)

The four methods used for separation of solids are:

Affinity purification

Filtration

Centrifugation

Crystallization

Yes, separation of solids and from liquid always necessary before extraction of fermentation products.

3)

Four methods of mechanical disruption of cells are:

Homogenization

Blending

freeze thawing

liquid shear

agitation with abrasives

Bead method

Cryopulverization

Microfluidizer Method

Nitrogen decompression

sonication

4)

Non-mechanical methods of cell disruption are:

Chemical Permeabilization

Mechanical Permeabilization

Enzymatic Permeabiization

Other Permeabilization Techniques

5)

Chemical agents that can be employed to extract fermentation products are:

Sulfuric acid

Methanol

Ethanol

Acetone

6)

Four methods of chromatography for purification of fermentation products are:

Adsorption chromatography

Affinity chromatography

Gel permeation chromatography

Reverse-phase chromatography

Ion-exchange chromatography

High-performance liquid chromatography

7)

The methods I would like to recommend for final processing of fermentation products before packaging are chromatography.

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