B. Making Acetate buffer. We will combine acetic acid (CH3COOH) and acetate ions

Question

B. Making Acetate buffer. We will combine acetic acid (CH3COOH) and acetate ions (CH3COO- ) in a proportion to make a buffer of pH 4.8. Calculate the amount of each stock solution and the amount of dH2O needed to prepare 50 mL of an acetate buffer that contains 20 mM sucrose, 40mM acetic acid, and 60 mM sodium acetate. Write your answers in the spaces below and copy to your lab rewrite.

Amount of 0.1 M sucrose solution needed ____________

Amount of 0.2 M acetic acid solution needed ____________

Amount of 0.2 M sodium acetate solution needed ____________

Amount of dH2O needed to bring to final volume ____________

Explanation / Answer

Acetate buffer =50ml , sucrose = 20mM, acetic acid =40mM, sodium acetate = 60 mM .formula for the caluculation for the dilutions will be ; c1v1=c2v2

amount of 0.1M sucrose solution = 100mM of sucorse needed ,amount of 0.2 =200 mM acetic acid , sodium acetate = 200mM,

Answers ;

Amount of 0.1 M sucrose solution needed = 20ml

Amount of 0.2M acetic acid = 20ml

Amount of 0.2 sodium acetate = 30 ml

Amount of dH20 needed = 30 ml

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