Enzymes have an optimum temperature at which they work best. Temperatures above
ID: 522776 • Letter: E
Question
Enzymes have an optimum temperature at which they work best. Temperatures above and below this optimum will decrease enzyme activity.
a) (1 point) Explain, on the molecular level, how temperature can affect enzyme activity.
c) (1 point) Based on our answers above, what structural level of the enzyme (primary, secondary, tertiary or quaternary) does the enzyme-substrate interaction most depend on?
2. (1 point) In the lab you will monitor the hydrolysis reaction of ONPG in the presence of beta-galactosidase. If you observe the reaction for 300 seconds and the absorbance changes from 0.003 to 0.578, calculate activity of beta-galactosidase (report in absorbance per minute).
Enzymes have an optimum temperature at which they work best. Temperatures above and below this optimum will decrease enzyme activity. Which graph best illustrates the effect of temperature on enzyme activity? c. Temperature A. Temperature a. A b. B c. C d. D B. Temperature D. Temperature Ans: B biotecniaExplanation / Answer
a) (1 point) Explain, on the molecular level, how temperature can affect enzyme activity.
Temperature will impact in the intermolecular level, the interactions will be either stronger or weaker, the activation site will be modified , thereofre no more substrate will be able to bind to the active site.
b) (1 point) Which graph best illustrates the effect of temperature on enzyme activity?
Temperature will initially increase the enzume activity and eventually achieve a peak, then it will decrease.
Best depiction must be B
c) (1 point) Based on our answers above, what structural level of the enzyme (primary, secondary, tertiary or quaternary) does the enzyme-substrate interaction most depend on?
The enzume must likely is tertiary, due to the fact taht 2 --> 3 (T favours) at T = 3, this is max, adn 3 --> 4 will decrease
2. (1 point) In the lab you will monitor the hydrolysis reaction of ONPG in the presence of beta-galactosidase. If you observe the reaction for 300 seconds and the absorbance changes from 0.003 to 0.578, calculate activity of beta-galactosidase (report in absorbance per minute).
t = 300 s = 300/60 = 5 min
dA/dT = (0.578-0.003 )/5= 0.115 /min
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.