Due Date: At the beginning of class; Friday, Sept 18 the 1. You are given a samp

Question

Due Date: At the beginning of class; Friday, Sept 18 the 1. You are given a sample of DNA that is the plasmid pxo1 from Bacillus for causative bacterium of the disease anthrax. The Pxol plasmid that carries the gene pairs, producing anthrax toxin is circular, is double-stranded, and contains 180,000 base with C content of 32.5%. following? (10 points) This pxoi plasmid contains how many of the a. deoxyribose residues b. purine nucleobases c. adenine nucleobases d. cytosine nucleobases e. hydrogen bonds

Explanation / Answer

This is given that G+C content is 32.5%

therefore, A+T will be = 100-32.5= 67.5%

According to chargaff rule A=T, And G=C

therefore A= T= 67.5/2=34.25%

G=C=32.5/2=16.25%

total no. of base pairs =180000= 360000 bases

therefore A=T= 33.75/100x360000=121500

And G=C=16.25/100x360000= 58500

A. no. of deoxyribose =no. of bases = 360000 because to every base there is one sugar moiety attached.

B. Purine nucleobase= A+G=58500+121500=180000

C.Adenine nucleobases= as per above calculastion

Adenine= 121500

D. Cytosine residue= 58500

E. Hydrogen bonds = there are two hydrogen bonds between purines

and three hydrogen bonds between pyrimidine

therefore, A bonded to T, hydrogen bonds= 121500x2= 243000.

bonds between pyrimidine= 58500x3=175500.

Total hydrogen bonds= 175500+243000=418500.

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