Due Date: At the beginning of class; Friday, Sept 18 You are given a sample of D

Question

Due Date: At the beginning of class; Friday, Sept 18 You are given a sample of DNA that is the plasmid pXOl from Bacillus anthracis, the causative bacterium of the disease anthrax. The Pxol plasmid that carries the gene for 1. producing anthrax toxin is circular, i ucing anthrax toxin is circular, is double-stranded, and contains 180,000 base pairs, with a G + C content of 32.5%. This pXOI plasmid contains how many of the following? (10 points) a. deoxyribose residues b. purine nucleobases c. adenine nucleobases d. cytosine nucleobases e. hydrogen bonds 2. Draw the structure of glycyl-seryl-cysteine. (5 points).

Explanation / Answer

a) 360000 bases are present in DNA

b) purine bases, According to Chargaff's rule %AT + % GC = 100; Here % GC = 32.5, Hence% AT = 100 - 32.5 = 67.5

Purine is Adenine and Guanine, Hence No of purine bases will be = (32.5/2 + 67.5/2) % = 33.75 + 16.25 = 50% Hence 180000 base pair of purine will be present.

c) Adenine - 33.75% = 121500 Adenine bases

d) Cytosine - 16.25% = 52000 cytosine bases

e) No of hydrogen bond is 180000 since 180000 bp is found

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