the success of PCR amplification depends on many factors one being that the prim
ID: 52489 • Letter: T
Question
the success of PCR amplification depends on many factors one being that the primers need to be in large excess to the template DNA to drive the reaction forward over many cycles, however looking at the reaction components the primers are used at only 2.5 times the mass amount of the template DNA explain this?
and give a reason for the lower than expected number of the smallest recombinant plasmid (2.82 kb)? this plasmid is constructed from pUC18 (2.69 kb) and the smallest lambda/hindIII fragment (0.13 kb).
Explanation / Answer
Answer:
PCR: Polymerase chain reaction
In the PCR reaction, The amplification of the DNA is done to increase content of DNA for the further studies, as the DNA extracted from the given organism is lower. The primer used in the reaction is made up of the small size ranging from 15 to 20 basepairs, there purpose is to start the reaction, so there amount is lower.
pUC 18 is a plasmid vector, where it can accomidate only the prokaryotes genes up to 15 kb in size, it is small size. only prokaryotes gene can be cloned.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.