17 of 38 General Chemistry 4th Edition University Science Books presented by Sap

Question

17 of 38 General Chemistry 4th Edition University Science Books presented by Sapling Leaming The data to the right were for the hydrolysis of ATP. Use these data to determine the values of Rmma (the maximum rate of the enzyme, often called the 67.0 950 maximum velocity, Vmaa) and KM, the Michaelis Menten 12.5 constant. Answer the four parts below. 20.0 119 435 155 166 DIf you were to plot this data to graphically determine R.we and KM using neweaver-Burk plot, what would you plot for the following data? If you would plot the original data, enter the original value in the ATP graph as graph as ol. L ol.L O pmol I 12.5 L. L. s. pmol 95.0 62.5 L. pmol L. s. pmol 166 Calculate the slope using the data points from part (a) Then determine the yintercept using the second point. b) AO Previous Give Up View Sokaion Check Answer 0 Next SExi

Explanation / Answer

1umole= 106picomoles

12.5 umoles/L= 12.5*106 picomole/L

hence ATP ( umole/L)     ATP    (picomoles/L) (S)                     1/S                                     1/R

12.5       12.5*106                    1/12.5*106 = 8*10-8                 =1/95=0.0105

the data is converted and shown in excel.

1/R=(KM/Rmax)*1/[ATP] +1/Rmax

Rmax is maximum rate and KM/Rmax is slope and 1/Rmax is intercept

from the 1/Rmax= 0.004, Rmax= 250 pmol/L.sec and slope is KM/Rmax = 74976, KM= 250*74976 pmole/L=1.9*107 picomole/L= 19 umole/L

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