Question No. 1 For a reaction that can take place with or without catalysis by a

Question

Question No. 1 For a reaction that can take place with or without catalysis by an enzyme, what would be the effect of the enzyme on the a. standard free energy change of the reaction? no cheoc b. activation energy of the reaction decrac initial velocity ofthe reaction? a etacts e ouse d. equilibrium constant of the reaction eRs no c Question No. 2 The turnover number for an enzyme is known to be 5000 min 1. rom the following set of data, determine the following without plotting or doing linear regression: Substrate concentration Initial velocity (mM) M/min 167 250 334 376 100 498 1000 499 a. vmax for the enzyme is b. Explain in one sentence how you determined vmax. bccurc w to low c. Kur for the enzyme is A d. Explain in one sentence how you determined KM e. Total enzyme-

Explanation / Answer

Ans 1.

a) The free energy associated with the reaction is difference of free energy of reactants from that of products.

The enzyme will not change the standard free energy of the reaction . As the energy is independent of the enzyme concentration .

b) The enzyme decreases the activation energy of the reaction , so that more number of reactants can cross the energy barrier and get converted into products .

c) The enzyme helps to convert more number of reactants into products , hence this increases the initial velocity of the reaction as more amount of product is formed.

d) equillibrium constant of the reaction K is given as

K = [products] / [reactants]

Hence it is not dependent on the concentration of enzyme.

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