The criteriafor acceptable biological and biochemical buffers were established b

Question

The criteriafor acceptable biological and biochemical buffers were established by studies conducted by Norman Good et al. in 1966. These criteria included a pKa Within physiological range soluble in water membrane impermeability minimal salt effedt minimal temperature and concentration effects on dissociation minimal interactions with cations chemical stability no/minimal, or well-documented, UV light absorbance at certain wavelengths easy to prepare Choose the buffer characteristic that fails to meet the criteria. Choose the best answer given the information NH2 pKa at 25 °C: 8.2 MW: 121.14 CH3 pK, at 25 : 4.76 MW: 142.085 Na CH2 CH2 Absorbance (260nm):0.06 Absorbance (280nm): 0.05 reactive with aldehydes Acetate buffer Michaelis buffer TRIS; Tris(hydroxymethyl)aminomethane O ineffective near physiological pH influence of temperature on minimal solubility in water soluble in nonpolar solvents O ineffective near physiological pH o influence of temperature on dissociation is too high dissociation is too high minimal solubility in water easily passes through cell membrane O

Explanation / Answer

Acetate buffer:ineffective near physiological PH

expalnation:

Tris buffer :infulence of temperature on dissociation is too high

explanation:

1. pKa declines approximately 0.03 units per degree Celsius rise in temperature.

2."ideal" biological buffer should have Minimal effects due to concentration or temperature

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