What types of amino acids do you predict will be present flanking a transmembran

Question

What types of amino acids do you predict will be present flanking a transmembrane helix? These amino acids are predicted to make interactions with the water and head groups of the membrane lipids. The following scheme was used to prepare membrane protein fractions separating integral and peripheral fractions. Cells were lysed in 10 mmol/L KCl for 15 minutes. The lysate was centrifuged to produce the S1 (supernatant 1) and the P1 (pellet 1) fractions. Soluble cytosolic proteins with the SI were removed by aspiration. The PI fraction was washed twice with fresh 10 mmol/L KCl followed by centrifugation each time. The washed PI was suspended in 500 mmol/L NaCl and agitated for 15 minutes. The suspension was centrifuged to produce the S2 and the P2 fractions. Peripheral membrane proteins were collected in the S2. The P2 was washed once with 500 mmol/L NaCl and centrifuged. The wash was pooled with the S2 fraction. The P2 was suspended in a non-denaturing detergent mixture (1% w/v N

Explanation / Answer

1c. The amino acids flanking the transmembrane helix will be polar amino acids. Since they interact with water and head groups of membrane lipids which are hydrophilic in nature therefore, they must also have polar nature.

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