1.A plasma sample was analyzed using ion selective electrodes (ISE) and the foll
ID: 53903 • Letter: 1
Question
1.A plasma sample was analyzed using ion selective electrodes (ISE) and the following electrolyte results obtained:
Sodium:140mM
Potassium:14.0mM
Chloride:112mM
Bicarbonate:18mM
These results are consistent with a specimen that:
Is severely lipemic
Is slightly hemolyzed
Was collected in an K+/EDTA tube
Needs to be diluted and reanalyzed
2.
This pathway is stimulated by insulin
Glycogenolysis
Glycolysis
Gluconeogenesis
Fermentation in the liver
3.
In the first step of the two-step approach to the diagnosis of gestational diabetes, the patient is given a glucose challenge dose of _____ g of glucose.
50
75
100
126
4.
This pathway is stimulated by Glucagon
Glycogenolysis
Glycolysis
Fermentation
Glycogenesis
5.
Which statement best describes the method of glucose determination by a polarographic electrode?
Increase of absorbance at 340 nm is directly proportional to glucose concentration in a sample
Decrease of absorbance at 340 nm is directly proportional to glucose concentration in a sample
Hydrogen peroxide generated in the reaction is utilized in a subsequent step to oxidize a chromagen (change dye color)
The rate decrease in partial pressure of oxygen in the reaction mixture is directly proportional to the concentration of glucose in the sample
a.Is severely lipemic
b.Is slightly hemolyzed
c.Was collected in an K+/EDTA tube
d.Needs to be diluted and reanalyzed
Explanation / Answer
1. The answer is B
The elctrolyte results suggested that sample is slightly hemolysed.
2. The answer is B
Insulin is a peptide hormone released from beta cells of pancreas in response to increased blood glucose levels. Its primary function is to remove the excess glucose from the circulation by metabolism through glycolysis, by uptake in liver in form of glycogen and its uptake in tissue.
3. The answer is A
Two step process involved the administartion of first, 50 gm dose followed by 100 gm dose to diagnose the gestational diabetes.
4. The anwer is A
Glucagon is a hormone released from alpha cells of pancreas in response to decreased blood glucose levels. glucagon increased blood glucose levels by inducing the catabolism of glycogen stored in liver through glycogenolysis and improve the falling glucose level.
5. The answer is C
hydrogen peroxide generated in the reaction is reacted with a dye (chromagen) in subsequant step and produces a specific color which is directly proportianal to the glucose concentration.
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