Consider temperate phage (a.k.a. “Fifi”). See the blue map of DNA as isolated fr

Question

Consider temperate phage (a.k.a. “Fifi”). See the blue   map of DNA as isolated from the phage capsid below. Like phage , integrates via itsattP into a unique attB in the E. coli chromosome, located between genes required to synthesize adenine and genes required to synthesize leucine (red map is region of host chromo where inserts). Note that attB is located much closer to leu than to ade.   possesses a cluster of genes essential for phage production (= A B C) and a cluster of non-essential genes (= 1 2 3).

Assuming that the attachment sites pair in the orientation shown in the above figures, draw the prophage map for ) . Your map must include ALL sites annotated in the above figures.

Explanation / Answer

The phage will insert between the genes of attB and the gene for adenine synthesis.

The gene order would be:

ade-A-B-C-attP-1-2-3-attB-leu

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