Consider temperate phage See the blue map of DNA as isolated from the phage caps

Question

Consider temperate phage See the blue map of DNA as isolated from the phage capsid below. Like phage lambda integrates via itsaatP into a unique attB in the E. coli chromosome, located between genes required to synthesize adenine and genes required to synthesize leucine (red map is region of host chromo where inserts). Note that attB is located much closer to leu than to ade. possesses a cluster of genes essential for phage production {= ABC) and a cluster of non-essential genes (= 1 2 3). Assuming that the attachment sites pair in the orientation shown in the above figures draw the prophage map for . Your map must include ALL sites annotated in the above figures.

Explanation / Answer

The map will be as follows:

ade....attL...1.....2.....3.....A.....B......C....attR.....leu

There will be a single crossover site specific recombination (between attachment sites attP and attB) via formation of Holliday junction which leads to a reversal of the sequence of genes.

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