4. You can base on the solubility, the charges, the hydrophobicity, the size, af

Question

4. You can base on the solubility, the charges, the hydrophobicity, the size, affinity, and ultracentrifugation, electrophoresis to separate and analyze proteins. (a). The flow rate of the conventional open chromatographic column is typical controlled by what? (b). The flow rate of the closed chromatographic column is typical controlled by what? What is HPLC standard for and what is its upper limited pressure? What is UPLC stand for and what is its upper limited pressure? (c). In ultracentrifugation, all proteins receive the same force to push them, what is the name of this force. What factors determine the sedimentation rate of the particles? (d). What is the significance of have a Ramachandran plot? In theory Phi and Psi angles are allowed to freely rotate, in reality it is impossible. Why? Why glycine and proline have high probability to locate in the turns of a globular protein?

Explanation / Answer

1.Equilibar regulators can be used as the primary flow control valve to avoid bulky globe valves and extend the flow control turndown range to 100:1

2.A method and apparatus for monitoring and controlling nano-scale flow rate of fluid in the operating flow path of a HPLC system provide fluid flow without relying on complex calibration routines to compensate for solvent compositions gradients typically used in HPLC. The apparatus and method are used to correct the flow output of a typical, analytical-scale (0.1-5 mL/min) HPLC pump to enable accurate anti precise flow delivery at capillary (<0.1 mL/min) and nano-scale (<L/min) HPLC flow rates.

3.Centrifugation is a process which involves the application of the centrifugal force for the sedimentation of heterogeneous mixtureswith a centrifuge

4.A Ramachandran plot can be used in two somewhat different ways. One is to show in theory which values, or conformations, of the and angles are possible for an amino-acid residue in a protein (as at top right a

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