Chromatin immunoprecipitation requires which of the following steps: proteolysis

Question

Chromatin immunoprecipitation requires which of the following steps:                 

                                proteolysis of cytoplasmic proteins and SDS-PAGE

                                crosslinking (i.e. fixation) DNA to nearby proteins and immunoprecipitation of a protein which binds DNA

                                crosslinking (i.e. fixation) DNA to mRNA and immunoprecipitation of mRNA which binds DNA

                                MALDI-TOF MS

                                proteolysis of cytoplasmic proteins and SDS-PAGE

                                crosslinking (i.e. fixation) DNA to nearby proteins and immunoprecipitation of a protein which binds DNA

                                crosslinking (i.e. fixation) DNA to mRNA and immunoprecipitation of mRNA which binds DNA

                                MALDI-TOF MS

Explanation / Answer

The possible step would be-

Crosslinking (i.e. fixation) DNA to nearby proteins and immunoprecipitation of a protein which binds DNA.

To check whether the protein binds to the DNA or not.

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