(Please provide photos of diagrams/maps!) QUESTION 3 Assume that you have at you

Question

(Please provide photos of diagrams/maps!)

QUESTION 3

Assume that you have at your disposal various deletion mutants of the phage and host genomes. Chose from the deletion list below those that can aid the recovery of ade+  transducing phage. Explain your choice(s).

deletions:                                        E. coli host deletions:

attP                                                      ade

A B C                                                  attB

1 2 3                                                    leu

                                                           ade through leu

                                                           DNA between ade and attB

DNA between leu and attB

QUESTION 4

Using your deletion trick in Question 3, you indeed isolate a viable ade+. Using a figure and words, show how you can now create a that can transduce bothade+ and leu+. Hint: Draw a ade+ chromosome next to a leu+ chromosome. Please draw this out.

Explanation / Answer

Based on the given data, question 1:

Question 3: From the above Phage lysogen, deletions that can aid the recovery of “ ade+” transducing phage is:

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