10/19/2017 11:55 PM A 4.1/10 10/18/2017 01:55 PM Gradebook Print Calculator Peri

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10/19/2017 11:55 PM A 4.1/10 10/18/2017 01:55 PM Gradebook Print Calculator Periodic Table Question 4 of 7 Map Sapling Learning An oligomeric protein has a molecular weight of 319000. A 930 mg sample was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) until the reaction was complete. Next the peptide bonds were hydrolyzed by heating with concentrated HCI. The product of the complete hydrolysis contained 1.5 of the compound shown below, and no other 2,4-dinitrophenyl derivatives of the -amino groups of r amino acids could be found. NO2 How many polypeptide chains are in this protein? (Enter your answer as a whole number). C-COOH CH3 Number Previous ( Give Up & View Solution O Check Answer Next H Exit Hint

Explanation / Answer

mmoles of 2,4 -DNP derivative = mass / molar mass

                                            = 1.5 mg / 256 g / mol

                                           = 5.86 x 10^-3 mmol

number of moles of protein = 930 / 319000

                                            = 2.92 x 10^-3 mmol

number of polypeptides bonds = 5.86 x 10^-3/ 2.92 x 10^-3

                                                 = 2.0

polypeptide chains = 2

                                                   

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