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1 . For each method, at what wavelengths should it be completed? To help answer

ID: 544534 • Letter: 1

Question

1. For each method, at what wavelengths should it be completed? To help answer this question, consider the spectra shown below in Figures 1 and 2, and the fact that the Lowry method produces a blue product.

Method

Wavelength for detection (nm)

Biuret

Lowry

Bradford

Figure 1. Spectra taken of different peptides using the Biuret assay.

Figure 2. Spectra taken from a Bradford assay of different concentrations of bovine serum albumin.

Method

Wavelength for detection (nm)

Biuret

Lowry

Bradford

0.15 0.1 0.05 400 500 600 Wavelength (nm) 700

Explanation / Answer

Method Wavelenght of detection (nm)

Biuret 540 nm

Lowry 750 nm

Bradford 465 and 595 nm

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