Also, answer the discussion questions below: Experiment 8: Post-Lab Discussion Q

Question

Also, answer the discussion questions below: Experiment 8: Post-Lab Discussion Questions: 1. Which is better suited to separate and detect small amounts of material: (a) 2. Compound X has an Rf value of 0.25. How far will compound X have traveled 3. How can you tell if different manufacturers used the same or different ink to 4. Based on the Rf values you obtained for the phospholipids, which of them column chromatography or (b) paper chromatography? Explain. from the origin when the eluent front has traveled 4 cm? produce the same color pens? migrates faster and which property influenced the rate of migration? Did it support your initial prediction? Consider the following compounds. Assuming these compounds can be separated by TLC, list them by increasing value of R 5. 112

Explanation / Answer

Ans 1 :

paper chromatography is better suited to seperate the coloured chemicals which are present in small amounts in a material. It is also used to test the purity of compounds and identifying the substances withing any material or compound. It is relatively a quicker procedure where the substances are seperated based on their affinity with the stationary and mobile phase.

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