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Researchers can manipulate the genes of a mouse so that a single gene in a singl

ID: 561365 • Letter: R

Question

Researchers can manipulate the genes of a mouse so that a single gene in a single tissue either produces an inactive protein (a knockout mouse) or produces a protein that is always (constitutively) active What effects on metabolism would you predict for mice with the following genetic changes? Knockout of the FBPase-2 domain of PFK-2/FBPase-2 in the liver: Effect on glucose metabolism: Effect on glucose levels: glycogenolysis suppressed elevated blood glucose lowered glucose glycolysis suppressed glycolysis stimulated Constitutively active PFK-2 in the liver: Effect on glucose metabolism: Effect on glucose levels:

Explanation / Answer

Biochemistry

Effect on metabolism predicted for mice,

Knockout of the FBPbase-2 domain of PFK-2/FBPase-2 in the liver:

Effect on glucose metabolism : Glycolysis stimulated

Effect on glucose level : lowered glucose

Constitutively active PFK-2 in the liver:

Effect on glucose metabolism : Glycolysis suppresed

Effect on glucose level : elevated blood glucose

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