You are interested in studying protein Y because it binds to your favorite prote

Question

You are interested in studying protein Y because it binds to your favorite protein X but only after X is covalently modified by a kinase Identify amino acids in Y that would be capable of forming ionic bonds with X after being modified by its kinase. You want to engineer a change in the amino acids of Y identified above in a) so that it binds to X after being modified by its kinase but with a lower affinity. Pick two amino acids that would have the smallest negative effect on binding. Protein X is an allosteric enzyme whose activity is positively regulated by binding to protein Y. Draw a graph below that shows the reaction rate of X on substrate S and identify the Km and Vmax when Y is absent Y is present

Explanation / Answer

a. Tyrosine Kinase as it has oH group that can mediate -VE charge on the amino acid and hene cxan form ionic bond.

b.Tyrosine and threonine as they are polar amino acid hence leads to lower soludility of deoxygegenated form and as aoverall result shows small negative effect of binding.

c.The particular ewnzyme activity is based on Michaelis-Mentn equation,a basic equation of enzyme kinetics.It represents arectangular hyperbola.

It has a basic concept that is :-

     V0 = Vmax (S) / KM + (S)

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