RNA polymerase II from eukaryotes has a carboxyl-terminal domain that is subject
ID: 56145 • Letter: R
Question
RNA polymerase II from eukaryotes has a carboxyl-terminal domain that is subject to phosphorylation by kinases in the cell. Two prominent modifications are to serine 2 and serine 5. Serine 5 peaks at the 5’ end of genes and serine 2 peaks at the 3’ ends of genes. You’ve identified a kinase from cells that you suspect modifies serine 2. You use a molecular technique called RNAi to deplete this kinase from cells. Do you think this would affect transcription initiation or capping? When you analyze the RNAs produced from the cells depleted of the kinase (accept that you can do this), you notice that the lengths of the RNAs in the depleted cells are abnormally long and have defects in polyadenylation. Why?
Explanation / Answer
5' end formation of pre-mRNAs is coupled transcription through the c-terminal domain of RNA polymersae II.
matured all protein coding by cleavage transcripts and by polyadenylation. misregulated frequently in disease, in human cells transcrption of coordinated with CPA is very important. the CTD is phosphorylated with Ser2 at poly A signals coincident with recruitment of the CstF77 CPA factor. 5' end mostly matured pre-mRNA are eukaryotic where they cleavage and polyadenylation. the only pre-mRNA transcritis not polyadenylated are those code for replication dependent histones.
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